Saliva Testing

Saliva testing is a convenient, inexpensive, and above all, accurate means of testing steroid hormones. Scientific studies have shown a strong correlation between steroid hormone levels in saliva and the amount of hormone in the blood that is active or "bioavailable." Saliva is an ideal diagnostic medium to measure the bioavailable levels of steroid hormones active in the tissue. It is this fraction of total hormone that is free to enter the target tissues in the brain, uterus, skin, and breasts.

Saliva testing can be done anywhere anytime. Testing that relies on blood drawn in the doctor's office makes it harder to obtain samples at specific times (such as in the early morning) or multiple times during the day. In addition, hormones in saliva are exceptionally stable and can be stored at room temperature for up to a week without affecting the accuracy of the result. This offers maximum flexibility in sample collection and shipment.
Best ValueBlood Spot and Saliva Tests are also available in ZRT's Combo Test Kit. Learn More
Hormones Tested in Saliva
Hormones we test in saliva include:

  • Estradiol (E2)
  • Estrone (E1)
  • Estriol (E3)
  • Progesterone (pg)
  • Testosterone (T)
  • DHEA-S (Ds)
  • Cortisol (C)

Reference and supplementation ranges:
- Saliva Reference Ranges
- Observed Salivary Supplementation Ranges
- ZRT Reference Range Determination
Profiles in Saliva
Profiles we offer in saliva include:

  • Female/Male Saliva Profile I
    (E2, Pg, T, DS, & C)
  • Female/Male Saliva Profile II
    (E2, Pg, T, DS, & C x 2)
  • Female/Male Saliva Profile III
    (E2, Pg, T, DS, & C x 4)

Compare Saliva and Blood Spot
The following table compares which tests are done in saliva and/or blood spot.

Hormone or Analyte


Blood Spot

Estradiol (E2)

Progesterone (Pg)

Testosterone, free


Testsoterone, total



Cortisol, AM (C1)

Cortisol, Diurnal (C1, C2, C3, C4)


Estriol (E3)


Estrone (E1)


Follicle stimulating hormone (FSH)


Lutenizing Hormone (LH)


Insulin-like Growth Factor (IGF-1)


Insulin, fasting


Triglycerides (TG)


Hemoglobin A1C (HbA1c)


High Sensitivity C-Reactive Protein (hs-CRP)


Total Cholesterol (CH)


LDL Cholesterol


HDL Cholesterol


VLDL Cholesterol


Prostate specific antigen (PSA)


Sex hormone binding globulin (SHBG)


Thyroid peroxidase antibodies (TPO)


Thyroid stimulating hormone (TSH)


Triiodothyronine (T3), free


Thyroxine (T4), free


25-hydroxy vitamin D2


25-hydroxy vitamin D3